ATP5MF (NM_004889) Human Recombinant Protein
Product Specifications
Background
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The catalytic portion of mitochondrial ATP synthase consists of five different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and single representatives of the gamma, delta, and epsilon subunits. The proton channel likely has nine subunits (a, b, c, d, e, f, g, F6 and 8) . This gene encodes the f subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has multiple pseudogenes. Naturally occurring read-through transcription also exists between this gene and the downstream pentatricopeptide repeat domain 1 (PTCD1) gene. [provided by RefSeq, Nov 2010]
Overview
Purified recombinant protein of Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F2 (ATP5J2), nuclear gene encoding mitochondrial protein, transcript variant 1, full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug
Synonyms
ATP5J2; ATP5JL
Gene ID
9551
UniProt
P56134
Accession Number mRNA
NM_004889
Chromosomal Location
7q22.1
Expression System
E. coli
Tag
N-GST and C-His
Related Pathways
Metabolic pathways, Oxidative phosphorylation
Field of Research
Metabolic pathways, Oxidative phosphorylation
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1% Sarkosyl.
Function
Transmembrane
Molecular Weight
38.7 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
ATP5MF
Species
Human
Protein ID
NP_004880
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/9551
Uniprot URL
https://www.uniprot.org/uniprot/P56134
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_004889
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_004880
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