MITF (NM_198158) Human Recombinant Protein

Product Specifications

Background

The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017]

Overview

Purified recombinant protein of Human microphthalmia-associated transcription factor (MITF), transcript variant 5, full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug

Synonyms

BHLHe32; CMM8; COMMAD; MI; WS2; WS2A

Gene ID

4286

UniProt

O75030

Accession Number mRNA

NM_198158

Chromosomal Location

3p13

Expression System

E. coli

Tag

N-GST and C-His

Related Pathways

Melanogenesis, Melanoma, Pathways in cancer

Field of Research

Melanogenesis, Melanoma, Pathways in cancer

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1% Sarkosyl.

Function

Druggable Genome, Transcription Factors

Molecular Weight

74.1 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.