MCM5 (NM_006739) Human Recombinant Protein

Product Specifications

Background

The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation. [provided by RefSeq, Jul 2008]

Overview

Purified recombinant protein of Human minichromosome maintenance complex component 5 (MCM5), full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug

Synonyms

CDC46; MGORS8; P1-CDC46

Gene ID

4174

UniProt

P33992, B1AHB0

Accession Number mRNA

NM_006739

Chromosomal Location

22q12.3

Expression System

E. coli

Tag

N-GST and C-His

Related Pathways

Cell cycle, DNA replication

Field of Research

Cell cycle, DNA replication

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1% Sarkosyl.

Function

Stem cell - Pluripotency, Transcription Factors

Molecular Weight

110.1 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.