Neutrophil Elastase (ELANE) (NM_001972) Human Recombinant Protein
Product Specifications
Background
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN) . This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016]
Synonyms
ELA2; GE; HLE; HNE; NE; PMN-E; SCN1
Gene ID
1991
UniProt
P08246
Accession Number mRNA
NM_001972
Chromosomal Location
19p13.3
Expression System
E. coli
Tag
N-GST and C-His
Related Pathways
Systemic lupus erythematosus
Field of Research
Systemic lupus erythematosus
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1%Sarkosyl.
Function
Protease, Transmembrane
Molecular Weight
53.5 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
ELANE
Species
Human
Protein ID
NP_001963
Overview
Purified recombinant protein ofHuman elastase, neutrophil expressed (ELANE), full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/1991
Uniprot URL
https://www.uniprot.org/uniprot/P08246
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_001972
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_001963
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