Neutrophil Elastase (ELANE) (NM_001972) Human Recombinant Protein

Product Specifications

Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN) . This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016]

Overview

Purified recombinant protein ofHuman elastase, neutrophil expressed (ELANE), full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug

Synonyms

ELA2; GE; HLE; HNE; NE; PMN-E; SCN1

Gene ID

1991

UniProt

P08246

Accession Number mRNA

NM_001972

Chromosomal Location

19p13.3

Expression System

E. coli

Tag

N-GST and C-His

Related Pathways

Systemic lupus erythematosus

Field of Research

Systemic lupus erythematosus

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1%Sarkosyl.

Function

Protease, Transmembrane

Molecular Weight

53.5 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.