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ARPC4 (NM_005718) Human Recombinant Protein

Product Specifications

Background

This gene encodes one of seven subunits of the human Arp2/3 protein complex. This complex controls actin polymerization in cells and has been conserved throughout eukaryotic evolution. This gene encodes the p20 subunit, which is necessary for actin nucleation and high-affinity binding to F-actin. Alternative splicing results in multiple transcript variants. Naturally occurring read-through transcription exists between this gene and the downstream tubulin tyrosine ligase-like family, member 3 (TTLL3), which results in the production of a fusion protein. [provided by RefSeq, Nov 2010]

Overview

Purified recombinant protein of Human actin related protein 2/3 complex, subunit 4, 20kDa (ARPC4), transcript variant 1, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug

Synonyms

ARC20; P20-ARC

Gene ID

10093

UniProt

P59998

Accession Number mRNA

NM_005718

Chromosomal Location

3p25.3

Expression System

E. coli

Tag

N-GST and C-His

Related Pathways

Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton

Field of Research

Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

50mM Tris, pH8.0, 8M Urea.

Molecular Weight

47.5 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

ARPC4

Species

Human

Protein ID

NP_005709

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/10093

Uniprot URL

https://www.uniprot.org/uniprot/P59998

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_005718

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_005709

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