ABHD4 (NM_022060) Human Recombinant Protein

Product Specifications

Background

Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE) . Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Human abhydrolase domain containing 4 (ABHD4), full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug

Synonyms

ABH4

Gene ID

63874

UniProt

Q8TB40

Accession Number mRNA

NM_022060

Chromosomal Location

14q11.2

Expression System

E. coli

Tag

N-GST, C-His

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1%Sarkosyl

Function

Protease

Molecular Weight

66.6 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.