H4C5 (NM_003545) Human Recombinant Protein
Product Specifications
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]
Synonyms
H4-16; H4/j; H4C1; H4C2; H4C3; H4C4; H4C6; H4C8; H4C9; H4C11; H4C12; H4C13; H4C14; H4C15; H4FJ; HIST1H4E
Gene ID
8367
UniProt
P62805, B2R4R0
Accession Number mRNA
NM_003545
Chromosomal Location
6p22.2
Expression System
E. coli
Tag
N-GST and C-His
Related Pathways
Systemic lupus erythematosus
Field of Research
Systemic lupus erythematosus
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1% Sarkosyl.
Molecular Weight
39.2 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
H4C5
Species
Human
Protein ID
NP_003536
Overview
Purified recombinant protein of Human histone cluster 1, H4e (HIST1H4E), full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/8367
Uniprot URL
https://www.uniprot.org/uniprot/P62805
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_003545
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_003536
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
