EIF4EBP1 (NM_004095) Human Recombinant Protein

Product Specifications

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]

Overview

Purified recombinant protein of Human eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1), Ser2-Ile118, with N-terminal His tag, expressed in E. coli, 50ug

Synonyms

4E-BP1; 4EBP1; BP-1; PHAS-I

Gene ID

1978

UniProt

Q13541

Accession Number mRNA

NM_004095

Chromosomal Location

8p11.23

Expression System

E. coli

Tag

N-His

Related Pathways

Acute myeloid leukemia, ErbB signaling pathway, Insulin signaling pathway, mTOR signaling pathway

Field of Research

Acute myeloid leukemia, ErbB signaling pathway, Insulin signaling pathway, mTOR signaling pathway

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1% Sarkosyl.

Molecular Weight

12.4 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.