DNMT1 Rabbit pAb (APR25881N)

Product Specifications

Background

This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.

Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality DNMT1 Rabbit pAb (APR25881N) .

Synonyms

ADCADN; AIM; CXXC9; DNMT; HSN1E; MCMT; m.HsaI; DNMT1

Gene ID

1786

UniProt

P26358

Cellular Locus

Nucleus

Applications

WB (Human hepatocellular carcinoma cell line, HUVEC, SMMC7721, BEL7404, Human cells, HEK293T, BEL-7402, Homo sapiens, Mus musculus, Rattus norvegicus) IF (Leporidae, Homo sapiens, Rattus norvegicus) IHC (Rattus norvegicus) ChIP (Homo sapiens)

Dilution

WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100

Form

Liquid

Buffer

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

Calculated MW: 144kDa/183kDa/184kDa Observed MW: 200kDa

Storage Conditions

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Gene ID URL

https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=1786

Uniprot URL

https://www.uniprot.org/uniprot/P26358

AA Sequence

MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKPEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGV