Acetyl-Histone H3-K27 Rabbit pAb (AMR00751N)
Product Specifications
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Synonyms
H3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
Gene ID
8290
UniProt
P68431
Cellular Locus
Chromosome, Nucleus
Applications
WB (Prawn gills, Danio rerio, Rattus norvegicus) ChIP (Mus musculus, Oryza sativa, Gallus gallus, Rattus norvegicus) ChIP-seq (Homo sapiens) ChIP-Seq (Zea mays) IF (Homo sapiens) IHC (Rattus norvegicus) IP (Rattus norvegicus) ChIP-qPCR (Rattus norvegicus)
Dilution
WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 ChIP-seq 1:20 - 1:100
Form
Liquid
Buffer
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Molecular Weight
Calculated MW: 15kDa Observed MW: 16kDa
Storage Conditions
Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Overview
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Acetyl-Histone H3-K27 Rabbit pAb (AMR00751N) .
Gene ID URL
https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=8290
Uniprot URL
https://www.uniprot.org/uniprot/P68431
AA Sequence
Email for sequence
Available Sizes
Curated Selection
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