UAP56 (DDX39B) Mouse Monoclonal Antibody [Clone ID: LBI8B8]

Product Specifications

Background

Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2; the effect of ALYREF/THOC4 is reported conflictingly with [PubMed:23299939] reporting a stimulatory effect. [UniProtKB/Swiss-Prot Function]

Overview

Carrier-free (BSA/glycerol-free) DDX39B mouse monoclonal antibody, clone LBI8B8

Synonyms

BAT1; D6S81E; UAP56

Gene Name

Homo sapiens DExD-box helicase 39B (DDX39B), transcript variant 1, mRNA.

Gene ID

7919

UniProt

Q13838

Conjugation

Unconjugated

Related Pathways

Spliceosome

Field of Research

Spliceosome

Dilution

WB 1:500, IHC 1:500

Form

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Buffer

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage Conditions

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.