Alexa Fluor™ 647-Labeled Human CD3 epsilon & CD3 delta Heterodimer Protein, His Tag&Tag FreeStar Staining

Alexa Fluor 647-Labeled Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (CDD-HA2W4) is produced via conjugation of AF647 to Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free with a new generation site-specific technology under Star Staining labeling platform. Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free is expressed from human 293 cells (HEK293) . It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D) ) .

Product Specifications

Background

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Specifications

Alexa Fluor 647-Labeled Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free, produced by co-expression of CD3E and CD3D. Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag.

Accession Number

P07766-1 & P04234-1

Expression Region

Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D)

Host

HEK293

Target

CD3E & CD3D

Conjugation

AF647 Labeled

Tag

C-10xHis | Native

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Alexa Fluor 647-Labeled Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-HA2W4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) (Cat. No. CDE-M531) with a linear range of 0.1-4 ng/mL (Routinely tested). Labeling with fluorescent dyes did not affect their activity.