SMAD2 Antibody (T220) [APR32425G]
Product Specifications
Background
Tissue Location: Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.
Synonyms
Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, JV18-1, Mad-related protein 2, hMAD-2, SMAD family member 2, SMAD 2, Smad2, hSMAD2, SMAD2, MADH2, MADR2
Gene Name
SMAD2
Gene ID
4087
UniProt
Q15796
Chromosomal Location
Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:9865696, PubMed:21145499) . On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081) . Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm at the blastocyst and epiblast stages (By similarity) {ECO:0000250|UniProtKB:Q62432, ECO:0000269|PubMed:16751101, ECO:0000269|PubMed:19289081, ECO:0000269|PubMed:21145499, ECO:0000269|PubMed:9865696}
Applications
IHC-P-Leica, WB
Dilution
IF--1:200 WB--1:1000
Form
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Function
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Molecular Weight
52306
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Overview
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SMAD2 Antibody (T220) [APR32425G].
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/?term=4087
Uniprot URL
https://www.uniprot.org/uniprot/Q15796
Available Sizes
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
MPC0645K Each
Magic™ Membrane Protein Human KCNV1 (Potassium voltage-gated channel modifier subfamily V member 1) Full Length
Ask
View Details 
MBS6385196-01 0.1 mL
MED18 (Mediator of RNA Polymerase II Transcription Subunit 18, Mediator Complex Subunit 18, p28b) (HRP)
Ask
View Details 
MBS6385196-02 5x 0.1 mL
MED18 (Mediator of RNA Polymerase II Transcription Subunit 18, Mediator Complex Subunit 18, p28b) (HRP)
Ask
View Details