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Rift Valley fever virus (RVFV) (strain MP12) glycoprotein / G1 Protein (His Tag)

Selected Variant

PO55450I2

Size: 100 µg

Product Specifications

Overview

A DNA sequence encoding the Rift Valley fever virus (RVFV) (strain MP12) GP (ABD38821.1) (Pro156-Thr581) was fused with a polyhistidine tag at the C-terminus.

Expression System

Baculovirus-Insect Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 85 % as determined by SDS-PAGE.

Activity

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Form

Lyophilized

Buffer

Lyophilized from sterile 20 mM PB, 300 mM NaCl, pH 7.0, 10 % glycerol. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

RVFV, Viral Proteins, Strain MP12

Molecular Weight

The recombinant Rift Valley fever virus (RVFV) (strain MP12) GP consists of 437 amino acids and predicts a molecular mass of 47.8 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

RVFV-G

Species

RVFV

Protein ID

ABD38821.1

AA Sequence

Pro156-Thr581

Protein ID Link

https://www.ncbi.nlm.nih.gov/protein/ABD38821.1

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