IdeS Protein, Streptococcus pyogenes, Recombinant (His & GST Tag)
Product Specifications
Background
IdeS (IgG-degrading enzyme of Streptococcus pyogenes) is a secreted cysteine endopeptidase from the human pathogen S. pyogenes with an extraordinarily high degree of substrate specificity, catalyzing a single proteolytic cleavage at the lower hinge of human IgG. This proteolytic degradation promotes inhibition of opsonophagocytosis and interferes with the killing of group A Streptococcus.
Overview
A DNA sequence encoding the streptococcus pyogenes IdeS (F8V4V0-1) (Asp30-Asn341) was expressed with the N-terminal polyhistidine-tagged a GST tag at the N-terminus (his-GST)
UniProt
F8V4V0-1
Expression System
E. coli
Tag
N-GST & His
Endotoxin
Please contact us for more information.
Purity
> 85 % as determined by SDS-PAGE.
Activity
One unit digests ≥ 95% of 1μg human IgG when incubated in PBS buffer, pH 7.4 at 37°C for 30min. The specific activity is 20,000 unit/mg.
Form
Liquid
Buffer
Supplied as sterile PBS, 50 % glycerol, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
Molecular Weight
The recombinant streptococcus pyogenes IdeS consists of 549 amino acids and predicts a molecular mass of 63.1 kDa.
Storage Conditions
Liquid. It is shipped out with blue ice. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
SD90_RS03345
Species
Streptococcus pyogenes
Uniprot URL
https://www.uniprot.org/uniprot/F8V4V0-1
AA Sequence
Asp30-Asn341
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