What sizes are available for IdeS Protein, Streptococcus pyogenes, Recombinant (His & GST Tag)?

IdeS Protein, Streptococcus pyogenes, Recombinant (His & GST Tag) is available in the following size(s): 20 KU.

IdeS Protein, Streptococcus pyogenes, Recombinant (His & GST Tag)

Q: What is the catalog number for IdeS Protein, Streptococcus pyogenes, Recombinant (His & GST Tag)?

The catalog number for IdeS Protein, Streptococcus pyogenes, Recombinant (His & GST Tag) is PO55274E6.

Product Specifications

Background

IdeS (IgG-degrading enzyme of Streptococcus pyogenes) is a secreted cysteine endopeptidase from the human pathogen S. pyogenes with an extraordinarily high degree of substrate specificity, catalyzing a single proteolytic cleavage at the lower hinge of human IgG. This proteolytic degradation promotes inhibition of opsonophagocytosis and interferes with the killing of group A Streptococcus.

Overview

A DNA sequence encoding the streptococcus pyogenes IdeS (F8V4V0-1) (Asp30-Asn341) was expressed with the N-terminal polyhistidine-tagged a GST tag at the N-terminus (his-GST)

UniProt

F8V4V0-1

Expression System

E. coli

Tag

N-GST & His

Endotoxin

Please contact us for more information.

Purity

> 85 % as determined by SDS-PAGE.

Activity

One unit digests ≥ 95% of 1μg human IgG when incubated in PBS buffer, pH 7.4 at 37°C for 30min. The specific activity is 20,000 unit/mg.

Form

Liquid

Buffer

Supplied as sterile PBS, 50 % glycerol, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

Molecular Weight

The recombinant streptococcus pyogenes IdeS consists of 549 amino acids and predicts a molecular mass of 63.1 kDa.

Storage Conditions

Liquid. It is shipped out with blue ice. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.