M.viridifaciens NA Protein, M.viridifaciens, Recombinant (His Tag)
Product Specifications
Synonyms
NA Protein, M.viridifaciens
Expression System
E. coli
Tag
N-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 97 % as determined by SDS-PAGE.
Activity
Measured by its ability to cleave a fluorogenic substrate, 2'- (4-Methylumbelliferyl) -α-D-N-acetylneuraminic acid. The specific activity is 10,000 pmoles/min/μg. as measured under the described conditions.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Molecular Weight
The recombinant M.viridifaciens neuraminidase consisting of 369 amino acids and has a calculated molecular mass of 39.7 kDa as estimated in SDS-PAGE under reducing conditions as predicted.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PO55044E1.pdf
Symbol
M. viridifaciens NA
Species
M.viridifaciens
Protein ID
BAA00852.1
Overview
A DNA sequence encoding the M.viridifaciens neuraminidase (BAA00852.1) (Pro 42-Gly 403) was expressed, with a N-terminal polyhistidine tag.
AA Sequence
Pro42-Gly403
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/BAA00852.1
Available Sizes
Curated Selection
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