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M.viridifaciens NA Protein, M.viridifaciens, Recombinant (His Tag)

Selected Variant

PO55044E1-01

Size: 10 µg

Product Specifications

Overview

A DNA sequence encoding the M.viridifaciens neuraminidase (BAA00852.1) (Pro 42-Gly 403) was expressed, with a N-terminal polyhistidine tag.

Synonyms

NA Protein, M.viridifaciens

Expression System

E. coli

Tag

N-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by SDS-PAGE.

Activity

Measured by its ability to cleave a fluorogenic substrate, 2'- (4-Methylumbelliferyl) -α-D-N-acetylneuraminic acid. The specific activity is 10,000 pmoles/min/μg. as measured under the described conditions.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Molecular Weight

The recombinant M.viridifaciens neuraminidase consisting of 369 amino acids and has a calculated molecular mass of 39.7 kDa as estimated in SDS-PAGE under reducing conditions as predicted.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

M. viridifaciens NA

Species

M.viridifaciens

Protein ID

BAA00852.1

AA Sequence

Pro42-Gly403

Protein ID Link

https://www.ncbi.nlm.nih.gov/protein/BAA00852.1

Available Sizes

Price

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