Her2/ERBB2 Protein, Rhesus, Recombinant (His Tag)

Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.

Measured by its binding ability in a functional ELISA. Immobilized Rhesus Her2/ERBB2 His (Cat:PK54222M2) at 2 μg/ml (100 μl/well) can bind Herceptin, the ECof Herceptin is 10-50 ng/mL.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Kinase proteins, Growth Factor proteins, CD Molecule proteins, CAR T Cell Therapy Target Proteins, Receptor Proteins, Cancer proteins, Immunotherapy proteins, Cytokine Proteins, Biomarker Proteins, Enzyme Proteins, Biological Target proteins, Diagnostic proteins

The recombinant Rhesus ErbB2 consists of 640 amino acids and predicts a molecular mass of 70.7 kDa. As a result of glycosylation, rhesus ErbB2 migrates as an approximately 105 kDa band in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.