NECTIN4/Nectin 4 Protein, Cynomolgus, Recombinant (ECD, hFc Tag)

Product Specifications

Overview

A DNA sequence encoding the Cynomolgus NECTIN4 (XP_005541277.1) (Met1-Ser349) was expressed with the fused Fc region of human IgG1 at the C-terminus.

Expression System

HEK293 Cells

Tag

C-hFc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Immobilized Recombinant Human CD111 / Nectin-1 / PVRL1 Protein (His Tag) (Cat:PH50410M2) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus NECTIN4 / Nectin 4 Protein (ECD, Fc Tag) (Cat:PK54220M4), the ECis 1.1-2.8 μg/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Molecular Weight

The recombinant Cynomolgus NECTIN4 consists of 556 amino acids and predicts a molecular mass of 60.79 kDa. It migrates as an approximately 72.8 kDa band in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.