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ENPP3 Protein, Rhesus, Recombinant (His Tag)

Product Specifications

Overview

A DNA sequence encoding the rhesus ENPP3 (XP_001103528.1) (Arg46-Ile874) was expressed with a polyhistide tag at the N-terminus.

Synonyms

ENPP3 Protein, Rhesus

Gene Name

Ectonucleotide pyrophosphatase/phosphodiesterase 3

Expression System

Baculovirus-Insect Cells

Tag

N-His

Field of Research

Phosphatases and Regulators

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Form

Lyophilized

Buffer

Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Enzyme Proteins

Molecular Weight

The recombinant rhesus ENPP3 consists of 845 amino acids and has a calculated molecular mass of 97.2 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

ENPP3

Species

Rhesus

Protein ID

XP_001103528.1

AA Sequence

Arg46-Ile874

Protein ID Link

https://www.ncbi.nlm.nih.gov/protein/XP_001103528.1

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