CD3E & CD3G Heterodimer Protein, Cynomolgus, Recombinant (Fc Tag)
Product Specifications
Gene Name
CD3e molecule, epsilon (CD3-TCR complex)
Expression System
HEK293 Cells
Tag
C-Fc
Endotoxin
< 0.01 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Molecular Weight
The recombinant cynomolgus CD3E/CD3G consists of 685 (345+340) amino acids and has a calculated molecular mass of 77.3 (38.8+38.5) KDa.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PK54207M4.pdf
Symbol
CD3E & CD3G
Species
Cynomolgus
Protein ID
Q95LI5.2&NP_001270839.1
Overview
A DNA sequence encoding the cynomolgus CD3E (Q95LI5.2) (Met1-Asp117) was fused with the Fc region of Llama IgG2b at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the cynomolgus CD3G (NP_001270839.1) (Met1-Thr113) was fused with the Fc region of Llama IgG2b at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the cynomolgus CD3E/CD3G heterodimer was purified.
AA Sequence
Met1-Asp117 & Met1-Thr113
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/Q95LI5.2&NP_001270839.1
Available Sizes
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