CD155/PVR Protein, Rhesus, Recombinant (His Tag)
Product Specifications
Background
CD155, commonly known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5), is a type I transmembrane single-span glycoprotein, and belongs to the nectins and nectin-like (Necl) subfamily. CD155 was originally identified based on its ability to mediate the cell attachment and entry of poliovirus (PV), an etiologic agent of the central nervous system disease poliomyelitis. The normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155 may assist in an efficient humoral immune response generated within the intestinal immune system. It has been demonstrated that CD155 can be recognized and bond by DNAM-1 and CD96 which promote the adhesion, migration and NK-cell killing, and thus efficiently prime cell-mediated tumor-specific immunity.
Overview
A DNA sequence encoding the rhesus PVR (NP_001036851.1) extracellular domain (Met 1-Asn 343) was expressed with a C-terminal polyhistidine tag.
Gene Name
Poliovirus receptor
Expression System
HEK293 Cells
Tag
C-His
Field of Research
Cancer Drug Targets
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 98 % as determined by SDS-PAGE.
Activity
1. Measured by its binding ability in a functional ELISA. Immobilized Rhesus CD155 at 2 μg/ml (100 μl/well) can bind human CD226 with a linear range of 1.28-32 ng/ml. 2. Immobilized TIGIT Protein, Human, Recombinant (mFc Tag) (Cat:PH51579M4) at 2 μg/ml (100 μl/well) can bind CD155/PVR Protein, Rhesus, Recombinant (His Tag) (Cat:PK54159M2), the ECof CD155/PVR Protein, Rhesus, Recombinant (His Tag) is 200-900 ng/mL.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
CD Molecule proteins, Cancer proteins, Immunotherapy proteins, Immune Checkpoint Proteins, Biological Target proteins
Molecular Weight
The recombinant rhesus PVR consists of 327 amino acids and has a calculated molecular mass of 35.8 kDa.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
PVR
Species
Rhesus
Protein ID
NP_001036851.1
AA Sequence
Met1-Asn343
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/NP_001036851.1
Available Sizes
Frequently Asked Questions
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