Her2/ERBB2 Protein, Cynomolgus, Recombinant (His Tag)

Product Specifications

Background

Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.

Overview

A DNA sequence encoding the cynomolgus ERBB2 (Met1-Thr652) [similar to Macaca fascicularis ERBB2 (EHH58073.1) ] was expressed with a polyhistidinetag at the C-terminus.

Gene Name

Erb-b2 receptor tyrosine kinase 2

Expression System

HEK293 Cells

Tag

C-His

Field of Research

Cancer Drug Targets Receptor Tyrosine Kinases (RTKs)

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

Immobilized Her2/ERBB2 Protein, Cynomolgus, Recombinant (His Tag) (Cat:PK54129M2) at 2 μg/ml (100 μl/well) can bind Anti-Erbb2 Antibody (Trastuzumab) (Cat:68046-H002), the ECis 6-40 ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Kinase proteins, Growth Factor proteins, CD Molecule proteins, CAR T Cell Therapy Target Proteins, Receptor Proteins, Cancer proteins, Immunotherapy proteins, Enzyme Proteins, Biological Target proteins, Diagnostic proteins, Cytokine Proteins, Biomarker Proteins

Molecular Weight

The recombinant cynomolgus ERBB2 comprises 641 amino acids and has a calculated molecular mass of 70.8 KDa. The apparent molecular mass of it is approximately 80-105 KDa in SDS-PAGE under reducing conditions due to glycosylation.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.