IL4R Protein, Cynomolgus, Recombinant (His Tag)

Product Specifications

Background

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alters the behavior of the cell. Some CD proteins do not take part in the cell signal process but have other functions such as cell adhesion. CD124, also known as the interleukin 4 receptor (IL4R), is a typeI transmembrane protein that can regulate IgE antibody production in B cells through binding to interleukin 4 and interleukin 13 and promote differentiation of Th2 cells through binding to interleukin 4. The membrane-bound form of CD124 can be hydrolyzed to a soluble form which can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells.

Overview

A DNA sequence encoding the cynomolgus IL4R (EHH60265.1) (Met1-Arg232) was expressed with a polyhistidine tag at the C-terminus.

Gene Name

Interleukin 4 receptor

Expression System

HEK293 Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

1. Immobilized IL4 (Cat:11846-HNAE) at 10 μg/mL (100 μL/well) can bind cynoIL4Rh (Cat:PK54118M2), the ECof cynoIL4Rh (Cat:PK54118M2) is 50-120 ng/mL. 2. Measured by its ability to inhibit IL-4 dependent proliferation of TF-1 human erthroleukemic cells. The EDfor this effect is 0.1-0.5ng/ml in the presence of 0.2 ng/ml IL-4.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

CD Molecule proteins, Receptor Proteins, Cytokine Proteins, Interleukin proteins

Molecular Weight

The recombinant cynomolgus IL4R consists of 218 amino acids and predicts a molecular mass of 25.2 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.