FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & AVI Tag), Biotinylated

A DNA sequence encoding the cynomolgus FCGRT (Q8SPV9) (Met 1-Ser 297) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the cynomolgus B2M (Q8SPW0) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Measured by its binding ability in a functional ELISA. Immobilized rhFc (CHO) (Cat:PH52028M0) at 10 μg/mL (100 μL/well) can bind cynoFCGRT-Avih (Cat:PK54103M9), the ECof cynoFCGRT-Avih (Cat:PK54103M9) is 400-900 ng/mL.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.