Ephrin A5 Protein, Rhesus, Recombinant (hFc Tag)

Product Specifications

Background

Ephrin-A5 also known as EFNA5, is a member of the Ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs) . Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A5/EFNA5 may function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Ephrin-A5/EFNA5 also serves as a cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.

Overview

A DNA sequence encoding the rhesus EFNA5 (NP_001252657.1) (Met 1-Asn 203) was fused with the Fc region of human IgG1 at the C-terminus.

Gene Name

Ephrin-A5

Expression System

HEK293 Cells

Tag

C-hFc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 92 % as determined by SDS-PAGE.

Activity

Measured by its binding ability in a functional ELISA.Immobilized Rhesus EFNA5-Fc (Cat:PK54035M4) at 10 μg/mL (100 μl/well) can bind biotinylated Rhesus EPHA4 (Cat:PK53941M4) . The ECof biotinylated Rhesus EPHA4 (Cat:PK53941M4) is 9.3-21.6 ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Cytokine Proteins, Growth Factor proteins, Enzyme Proteins, Biological Target proteins

Molecular Weight

The recombinant rhesus EFNA5 consists of 424 amino acids and has a calculated molecular mass of 48.2 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.