PDGFRB Protein, Rhesus, Recombinant (hFc Tag)

Product Specifications

Background

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD140b, also known as PDGFRB, is a member of the CD system. CD140b is a cell surface tyrosine kinase receptor essencial for development interacting with the platelet-derived growth factors (PDGFs) which serves as mitogens for mesenchymal cells. CD140b can bind with platelet-derived growth factor (PDGF) -B, that are secreted by tumors and phosphorylation of PDGFR-β was correlated with depth of cancer invasion at statistically significant level.

Overview

A DNA sequence encoding the rhesus PDGFRB (XP_001107595.1) (Met1-Phe530) was expressed with the Fc region of human IgG1 at the C-terminus.

Gene Name

Platelet-derived growth factor receptor, beta polypeptide

Expression System

HEK293 Cells

Tag

C-hFc

Field of Research

Cancer Drug Targets Receptor Tyrosine Kinases (RTKs)

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 85 % as determined by SDS-PAGE.

Activity

1.Measured by its binding ability in a functional ELISA. 2.Immobilized mouse PDGF-B (Cat:PM53450Y1) at 10μg/mL (100μL/well) can bind rhesus PDGFRB (Cat:PK53954M4), the ECof rhesus PDGFRB is 0.02-0.2 μg/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Kinase proteins, Diagnostic proteins, Growth Factor proteins, CD Molecule proteins, Receptor Proteins, Cancer proteins, Enzyme Proteins, Cytokine Proteins, Biomarker Proteins

Molecular Weight

The recombinant rhesus PDGFRB consists 739 amino acids and predicts a molecular mass of 83.3 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.