IGFBP5 Protein, Cynomolgus, Recombinant (His Tag)
Product Specifications
Gene Name
Insulin-like growth factor binding protein 5
UniProt
F1PEQ5
Expression System
HEK293 Cells
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 90 % as determined by SDS-PAGE.
Activity
Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF-7 human breast cancer cells. The EDfor this effect is 1-6 μg/mL in the presence of 14 ng/mL rhIGF-II.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
Growth Factor proteins
Molecular Weight
The recombinant cynomolgus IGFBP5 comprises 263 amino acids and has a calculated molecular mass of 30 KDa. The apparent molecular mass of it is approximately 38 and 23 KDa in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PK53927M2.pdf
Symbol
IGFBP5
Species
Cynomolgus
Overview
A DNA sequence encoding the cynomolgus IGFBP5 (F1PEQ5) (Met1-Glu271) was expressed with a polyhistidine tag at the C-terminus.
Uniprot URL
https://www.uniprot.org/uniprot/F1PEQ5
AA Sequence
Met1-Glu271
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