Frizzled 4 Protein, Rat, Recombinant (hFc Tag)
Product Specifications
Background
Familial exudative vitreoretinopathy (FEVR) is a hereditary blinding disorder that features defects in retinal vascular development. Mutations in FZD4 are known to cause autosomal dominant exudative vitreoretinopathy (EVR1) . The mutations in FZD4 are associated with the phenotypes of retinal folds or ectopic macula in FEVR
Gene Name
Frizzled class receptor 4
UniProt
Q9QZH0
Expression System
HEK293 Cells
Tag
C-hFc
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
Growth Factor proteins, CD Molecule proteins, Receptor Proteins, Cytokine Proteins, epigenetic proteins
Molecular Weight
The recombinant rat FZD4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 385 amino acids and has a predicted molecular mass of 43.2 kDa. The apparent molecular mass of the protein is approximately 113, 58 and 36 kDa in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PR53766M4.pdf
Symbol
FZD4
Species
Rat
Overview
A DNA sequence encoding the rat FZD4 (Q9QZH0) (Met1-Glu181) was expressed with the Fc region of human IgG1 at the C-terminus.
Uniprot URL
https://www.uniprot.org/uniprot/Q9QZH0
AA Sequence
Met1-Glu181
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