PDGFRB Protein, Rat, Recombinant (His Tag)

Product Specifications

Background

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD140b, also known as PDGFRB, is a member of the CD system. CD140b is a cell surface tyrosine kinase receptor essencial for development interacting with the platelet-derived growth factors (PDGFs) which serves as mitogens for mesenchymal cells. CD140b can bind with platelet-derived growth factor (PDGF) -B, that are secreted by tumors and phosphorylation of PDGFR-β was correlated with depth of cancer invasion at statistically significant level.

Overview

A DNA sequence encoding the rat PDGFRB (Q05030) (Met1-Lys530) was expressed, fused with a polyhistidine tag at the C-terminus.

Gene Name

Platelet-derived growth factor receptor, beta polypeptide

UniProt

Q05030

Expression System

HEK293 Cells

Tag

C-His

Field of Research

Cancer Drug Targets Receptor Tyrosine Kinases (RTKs)

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured by its binding ability in a functional ELISA. Immobilized rat PDGFRB-His at 10 μg/ml (100 μl/well) can bind Cynomolgus PDGFB (Cat:90030-C04H), The ECof Cynomolgus PDGFB (Cat:90030-C04H) is 3-7 ng/ml.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Kinase proteins, Diagnostic proteins, Growth Factor proteins, CD Molecule proteins, Receptor Proteins, Cancer proteins, Enzyme Proteins, Biomarker Proteins, Cytokine Proteins

Molecular Weight

The recombinant rat PDGFRB comprises 510 amino acids and predicts a molecular mass of 57.6 kDa. The apparent molecular mass of the recombinant protein is approximately 92 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.