TWEAK/TNFSF12 Protein, Rat, Recombinant (hFc Tag)
Product Specifications
Background
TNFSF12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. It can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. It is also found that TNFSF12 promotes proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. TNFSF12 also is a weak inducer of apoptosis in some cell types and mediates NF-kappa-B activation.
Gene Name
Tumor necrosis factor (ligand) superfamily, member 12
Expression System
HEK293 Cells
Tag
N-hFc
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 85 % as determined by SDS-PAGE.
Activity
Immobilized Fc-rat TNFSF12 at 10 μg/ml (100 μl/well) can bind biotinylated human Fc-TNFRSF12A (Cat:PH50952M3), The ECof biotinylated human Fc-TNFRSF12A (Cat:PH50952M3) is 0.12-0.28 μg/ml.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
Cytokine Proteins, Growth Factor proteins, TNF Superfamily proteins, Immunotherapy proteins, Biological Target proteins
Molecular Weight
The recombinant rat TNFSF12 comprises 405 amino acids and predicts a molecular mass of 44.4 kDa. The apparent molecular mass of the recombinant protein is approximately 49 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PR53624M3.pdf
Symbol
TNFSF12
Species
Rat
Protein ID
NP_001001513.2
Overview
A DNA sequence encoding the rat TNFSF12 (NP_001001513.2) (Arg105-His249) was expressed with Fc region of human IgG1 at the N-terminus.
AA Sequence
Arg105-His249
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/NP_001001513.2
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