Nectin-2 Protein, Mouse, Recombinant (His Tag)

Product Specifications

Background

Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.

Overview

A DNA sequence encoding the mouse PVRL2 (NP_033016.3) extracellular domain (Met 1-Gly 351) was fused with a polyhistidine tag at the C-terminus.

Synonyms

AI325026 Protein, Mouse; AI987993 Protein, Mouse; Cd112 Protein, Mouse; MPH Protein, Mouse; nectin-2 Protein, Mouse; Pvr Protein, Mouse; Pvs Protein, Mouse

Gene Name

Poliovirus receptor-related 2 (herpesvirus entry mediator B)

Expression System

HEK293 Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by SDS-PAGE.

Activity

Measured by its binding ability in a functional ELISA.1. Immobilized mouse CD112-His (Cat: PM53534M2) at 10 μg/ml (100 μl/well) can bind biotinylated mouse DNAM1-His (Cat: PM53327M2) with a linear range of 0.156-5.0 μg/ml. 2. Immobilized mouse CD112-His (Cat: PM53534M2) at 10 μg/ml (100 μl/well) can bind mouse DNAM1-Fc (Cat: 50232-M03H) with a linear range of 0.03-1.0 μg/ml.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

CD Molecule proteins, Immune Checkpoint Proteins, Biological Target proteins

Molecular Weight

The secreted recombinant mouse PVRL2 comprises 331 amino acids with a predicted molecular mass of 36 kDa. As a result of glycosylation, it migrates as an approximately 40-45 kDa band in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.