ENTPD3 Protein, Mouse, Recombinant (His Tag)

Product Specifications

Overview

A DNA sequence encoding the mouse ENTPD3 (NP_848791.3) (Gln44-Pro485) was expressed with a polyhistidine tag at the C-terminus.

Synonyms

Cd39l3 Protein, Mouse; HB6 Protein, Mouse; NTPDase-3 Protein, Mouse

Gene Name

Ectonucleoside triphosphate diphosphohydrolase 3

Expression System

Baculovirus-Insect Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Form

Lyophilized

Buffer

Lyophilized from sterile 20 mM Tris, 300 mM NaCl, pH 7.5, 10 % glycerol. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Molecular Weight

The recombinant mouse ENTPD3 consists of 453 amino acids and predicts a molecular mass of 50.9 kDa. It migrates as an approximately 55.8 kDa band in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.