SerpinA3c Protein, Mouse, Recombinant (His Tag)

1A1 Protein, Mouse; Kalbp Protein, Mouse; Klkbp Protein, Mouse; MGC144822 Protein, Mouse; Serpin A3C Protein, Mouse; Serpina3c Protein, Mouse; spi2 Protein, Mouse

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The secreted recombinant mouse SERPINA3C comprises 406 amino acids with a predicted molecular mass of 46 kDa. As a result of diiferent glycosylation, the apparent molecular mass of rm SERPINA3C is approximately 50-60 kDa and 110 kDa in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

A DNA sequence encoding the extracellular domain of mouse SERPINA3C (NP_032484.1) precursor (Met 1-Ala 417) was expressed, with a C-terminal polyhistidine tag.