Methionyl aminopeptidase 2/METAP2 Protein, Mouse, Recombinant (His Tag)

METAP2 (Methionine aminopeptidase 2), also known as MAP2 is a protein that belongs to the peptidase M24A family. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. The catalytic activity of human MAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis.

4930584B20Rik Protein, Mouse; A930035J23Rik Protein, Mouse; AI047573 Protein, Mouse; AL024412 Protein, Mouse; Amp2 Protein, Mouse; AU014659 Protein, Mouse; Mnpep Protein, Mouse; p67 Protein, Mouse; p67eIF2 Protein, Mouse

Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC (Catalog # ES017) . The resulting GP-AMC is cleaved by Recombinant Human DPPIV/CD26 (Catalog # 1180-SE) . The specific activity is >15 pmol/min/μg.

Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

The recombinant mouse METAP2 consists of 488 amino acids and has a calculated molecular mass of 54.3 kDa. It migrates as an approximately 60 KDa band in SDS-PAGE under reducing conditions.

Liquid. It is shipped out with blue ice. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.