CD86 Protein, Mouse, Recombinant (His Tag)

CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. A recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.

B7 Protein, Mouse; B7-2 Protein, Mouse; B7.2 Protein, Mouse; B70 Protein, Mouse; Cd28l2 Protein, Mouse; CLS1 Protein, Mouse; ETC-1 Protein, Mouse; Ly-58 Protein, Mouse; Ly58 Protein, Mouse; MB7 Protein, Mouse; MB7-2 Protein, Mouse; TS/A-2 Protein, Mouse

Measured by its binding ability in a functional ELISA. Immobilized mouse CD86 at 20 μg/ml (100 μl/well) can bind mouse CD28 with a linear range of 6.4-800 ng/ml.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

B7 Family proteins, CD Molecule proteins, Cancer proteins, Immunotherapy proteins, Immune Checkpoint Proteins, Biological Target proteins, Diagnostic proteins

The recombinant mouse CD86 consists of 233 amino acids after removal of the signal peptide and has a predicted molecular mass of 26.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD86 is approximately 40-55 kDa due to glycosylation.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

A DNA sequence encoding the extracellular domain (Met 1-Glu 245) of mouse CD86 (NP_062261.3) precursor was fused with a polyhistidine tag at the C-terminus.