CD147 Protein, Mouse, Recombinant (His & hFc Tag)

Product Specifications

Background

CD147/EMMPRIN (Extracellular Matrix Metalloproteinase Inducer), also known as Basigin (BSG), is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. It is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including spermatogenesis, embryo implantation, neural network formation. CD147 induces the production and release of matrix metalloproteinases (MMP) in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction. Recently, CD147 displays increased expression in many cancers, and it has been previously demonstrated to participate in cancer metastasis and progression. Thus, CD147 and its antibody are used as an effective treatment for malignant cancers.

Overview

A DNA sequence encoding the extracellular domain of mouse BSG isoform 2 (NP_001070652.1) (Met 1-Arg 209) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Synonyms

AI115436 Protein, Mouse; AI325119 Protein, Mouse; CD147 Protein, Mouse; EMMPRIN Protein, Mouse; HT-7 Protein, Mouse

Gene Name

Basigin (Ok blood group)

Expression System

HEK293 Cells

Tag

C-hFc & His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

Using the Octet RED System, the affinity constant (Kd) of mouse CD147-Fc (Cat:PM53381M4) bound to Human PPIA-His (Cat:PH50617E2) was 0.4 μM.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

CD Molecule proteins, Diagnostic proteins, Biomarker Proteins, Enzyme Proteins

Molecular Weight

The secreted recombinant mouse BSG/Fc is a disulfide-linked homodimer The reduced monomer comprises 436 amino acids and has a predicted molecular mass of 48.6 kDa. As a result of glycosylation, it migrates as an approximately 60-70 kDa band in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.