Serpina1d Protein, Mouse, Recombinant

Product Specifications

Overview

A DNA sequence encoding the mouse Serpina1a (XP_003945711.1) (Met1-Lys413) was expressed with five amino acids (DDDDK) at the C-terminus.

Synonyms

AI118301 Protein, Mouse; Dom4 Protein, Mouse; PI4 Protein, Mouse; Serpin A1d Protein, Mouse; Spi1-4 Protein, Mouse

Gene Name

Serine (or cysteine) peptidase inhibitor, clade A, member 1D

Expression System

HEK293 Cells

Tag

No tag

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by SDS-PAGE.

Activity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-Arg-Pro-Lys-Pro-Val-Glu-Nval-Trp-Arg-Lys (DNP) -NH2.The IC50 value is < 15 nM.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Enzyme Proteins

Molecular Weight

The recombinant mouse Serpina1a comprises 395 amino acids and has a predicted molecular mass of 44.1 kDa. The apparent molecular mass of the protein is approximately 54 kDa in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.