EphA4 Protein, Mouse, Recombinant (hFc Tag)

EPH receptor A4 (ephrin type-A receptor 4), also known as EphA4, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. EphA4 is enriched on dendritic spines of pyramidal neurons in the adult mouse hippocampus, and ephrin-A3 is localized on astrocytic processes that envelop spines. Eph receptor mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs) . The extracellular domain of an EphA4 interacts with ephrin ligands, which may be tethered to neighbouring cells. Ligand-mediated activation of Ephs induces various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer.

2900005C20Rik Protein, Mouse; AI385584 Protein, Mouse; Cek8 Protein, Mouse; Hek8 Protein, Mouse; rb Protein, Mouse; Sek Protein, Mouse; Sek1 Protein, Mouse; Tyro1 Protein, Mouse

Measured by its binding ability in a functional ELISA. Immobilized mouse EPHA5 at 2 μg/ml (100 μl/well) can bind mouse EFNA4-Fc with a linear range of 1.28-32 ng/ml.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The recombinant mouse EPHA4/Fc is a disulfide-linked homodimer. The reduced monomer consists of 768 amino acids and has a predicted molecular mass of 85.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmEPHA4/Fc monomer is approximately 110 kDa due to glycosylation.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

A DNA sequence encoding the mouse EPHA4 (NP_031962.2) extracellular domain (Met 1-Thr 547) was as fused with the Fc region of human IgG1 at the C-terminus.