FCGRT & B2M Heterodimer Protein, Mouse, Recombinant (His Tag)

A DNA sequence encoding the extracellular domain (Met 1-Ser 297) of mouse FCGRT (Q61559) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the mouse B2M (P01887,105Ala/Asp) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified

1. Measured by its ability to bind human IgG1 in functional ELISA. 2. Labeled biotin to FCGRT & B2M Heterodimer Protein, Mouse, Recombinant (His Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of FCGRT & B2M Heterodimer Protein, Mouse, Recombinant (His Tag), Biotinylated (Cat. PM52825M2) bound to IgG1 Antibody was 1.6 nM.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The recombinant heterodimer of mouse FCGRT / B2M comprises 385 (285 + 100) amino acids and has a calculated molecular mass of 43.5 (31.8 + 11.7) kDa. The apparent molecular mass of FCGRT / B2M heterodimer is approximately 35 & 12 kDa respectively in SDS-PAGE under reducing conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.