What is the catalog number for ICOS ligand Protein, Mouse, Recombinant (His Tag)?

The catalog number for ICOS ligand Protein, Mouse, Recombinant (His Tag) is PM53329M2.

What sizes are available for ICOS ligand Protein, Mouse, Recombinant (His Tag)?

ICOS ligand Protein, Mouse, Recombinant (His Tag) is available in the following size(s): 100 µg, 1 mg.

How many pack sizes does ICOS ligand Protein, Mouse, Recombinant (His Tag) come in?

ICOS ligand Protein, Mouse, Recombinant (His Tag) is available in 2 different pack sizes.

Is ICOS ligand Protein, Mouse, Recombinant (His Tag) in stock?

ICOS ligand Protein, Mouse, Recombinant (His Tag) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

ICOS ligand Protein, Mouse, Recombinant (His Tag)

Product Specifications

Background

Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. The present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production, and germinal center formation.

Overview

A DNA sequence encoding the mouse B7-H2 (NP_056605.1) extracellular domain (Met 1-Lys 279) was fused with a polyhistidine tag at the C-terminus.

Synonyms

B7-H2 Protein, Mouse; B7h Protein, Mouse; B7RP-1 Protein, Mouse; GI50 Protein, Mouse; GL50 Protein, Mouse; GL50-B Protein, Mouse; ICOS-L Protein, Mouse; Icoslg Protein, Mouse; LICOS Protein, Mouse

Gene Name

Inducible T-cell co-stimulator ligand

Expression System

HEK293 Cells

Tag

C-His

Field of Research

Cancer Drug Targets

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured by its binding ability in a functional ELISA. Immobilized mouse B7-H2 at 1 μg/ml (100 μl/well) can bind human ICOS with a linear range of 40-1000 ng/ml.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

B7 Family proteins, CD Molecule proteins, Cancer proteins, Immunotherapy proteins, Immune Checkpoint Proteins, Biological Target proteins

Molecular Weight

The recombinant mouse B7-H2 comprises 244 amino acids with a predicted molecular mass of 27.8 kDa. As a result of glycosylation, the apparent molecular mass of rmB7-H2 is approximately 45-55 kDa in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.