Renin Protein, Mouse, Recombinant (His Tag)

Renin-1, also known as Ren-1, Angiotensinogenase and Kidney renin, is a member of the peptidase A1 family. Renin-1 is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration. androgen and thyroid hormones influence levels of Renin-1 in mouse submandibular gland (SMG) primarily by regulating the amount of Renin-1 mRNA available for translation. Renin-1 is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It is expressed at relatively low levels in mouse SMG and kidney. Ren-2 is expressed at high levels in the mouse SMG and at very low levels, if at all, in the kidney. Ren-1 and Ren-2 are closely linked on mouse chromosome 1, show extensive homology in coding and noncoding regions and provide a model for studying the regulation of gene expression.

D19352 Protein, Mouse; Ren Protein, Mouse; Ren-1 Protein, Mouse; Ren-2 Protein, Mouse; Ren-A Protein, Mouse; Ren-B Protein, Mouse; Ren1 Protein, Mouse; Ren1c Protein, Mouse; Ren1d Protein, Mouse; Ren2 Protein, Mouse; Rn-1 Protein, Mouse; Rn-2 Protein, Mouse; Rnr Protein, Mouse

1. Measured by its ability to cleave the fluorogenic peptide substrate 5-FAM/QXL 520 (Peti-Peterdi, J. et al., 2009, Physiology 24:88.) . The specific activity is > 20 pmoles/min/μg. 2. Immobilized mouse REN1-His (Cat: PM53324M2) at 10ug/ml (100 μl/well) can bind biotinylated human AGT-His (Cat: PH51255M2) with a linear range of 31.25-250 ng/ml.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The recombinant mouse REN1 consists of 392 amino acids and has a predicted molecular mass of 43.2 kDa. As a result of glycosylation, the apparent molecular mass of rmREN1 is approximately 48-55 kDa in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.