Protein C Protein, Mouse, Recombinant (His Tag)

Product Specifications

Background

PROC (protein C gene) is a 62-kDa vitamin K-dependent plasma zymogen which, after activation to serine protease, plays an important role in the physiologic regulation of blood coagulation. Originally thought to be synthesized exclusively by the liver, recent reports have shown that PROC is also produced by many other cells including pancreatic islet β cells. Protein C a vitamin K-dependent plasma glycoprotein and is one of the body’s natural anticoagulants circulating at 70 nmol/L as an inactive protease zymogen.

Overview

A DNA sequence encoding the mouse PROC (NP_032960.3) (Met1-Leu460) was expressed with a C-terminal polyhistidine tag.

Synonyms

PC Protein, Mouse; PROC Protein, Mouse

Gene Name

Protein C, inactivator of coagulation factors Va and VIIIa

Expression System

HEK293 Cells

Tag

C-His

Field of Research

Serine Proteases and Regulators

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

>85 % as determined by SDS-PAGE.

Activity

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Molecular Weight

The recombinant mouse PROC comprises 453 amino acids and has a predicted molecular mass of 51.1 kDa. The apparent molecular mass of the protein is approximately 63 and 45 kDa in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.