Ephrin A5 Protein, Mouse, Recombinant (His Tag)

Ephrin-A5 also known as EFNA5, is a member of the Ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs) . Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A5/EFNA5 may function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Ephrin-A5/EFNA5 also serves as a cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.

A DNA sequence encoding the mouse EFNA5 isoform long (O08543-1) without the pro peptide (Met 1-Asn 203) was expressed, with a polyhistidine tag at the C-terminus.

AL-1 Protein, Mouse; AV158822 Protein, Mouse; EFL-5 Protein, Mouse; Ephrin-A5 Protein, Mouse; Epl7 Protein, Mouse; LERK-7 Protein, Mouse; RAGS Protein, Mouse

Measured by its binding ability in a functional ELISA. Immobilized Mouse EPHA5 at 2 μg/ml (100 μl/well) can bind mouse EFNA4 with a linear range of 1.28-32 ng/ml.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The recombinant mouse EFNA5 consists of 194 amino acids and has a predicted molecular mass of 22.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmEFNA5 is approximately 28 kDa due to glycosylation.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.