C-MET Protein, Mouse, Recombinant (ECD, His Tag)

Product Specifications

Background

Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that is overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair, or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.

Overview

A DNA sequence encoding the mouse MET (EDL13881.1) extracellular domain (Met 1-Asn 929) was fused with a polyhistidine tag at the C-terminus.

Synonyms

AI838057 Protein, Mouse; c-Met Protein, Mouse; HGF Protein, Mouse; HGFR Protein, Mouse; Par4 Protein, Mouse

Gene Name

MET proto-oncogene, receptor tyrosine kinase

Expression System

HEK293 Cells

Tag

C-His

Field of Research

Cancer Drug Targets Receptor Tyrosine Kinases (RTKs)

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

Immobilized HGF Protein, Mouse, Recombinant (Cat:50038-MNAH) at 2 μg/mL (100 μL/well) can bind c-MET Protein, Mouse, Recombinant (ECD, His Tag) (Cat:PM53214M2), the ECis 80-400 ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Kinase proteins, CAR T Cell Therapy Target Proteins, Receptor Proteins, Cancer proteins, Immunotherapy proteins, Biomarker Proteins, epigenetic proteins, Enzyme Proteins, Biological Target proteins, Diagnostic proteins

Molecular Weight

The recombinant mouse Met is a heterodimer composed of the proteolytically cleaved α and β subunits. The α and β heterodimer consists of 916 amino acids and has a predicted molecular mass of 102 (α =32 + β=70) kDa. The apparent molecular mass of the rmMET heterodimer thus is approximately 41.8 kDa and 87.2 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.