CD200R Protein, Mouse, Recombinant (His & hFc Tag)

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cell surface glycoprotein CD200 receptor 1 (CD200R1) is an isoform of CD200 receptors that is expressed on cells of the myeloid lineage. CD200R1 is a receptor for the OX-2 membrane glycoprotein. The receptor-substrate interaction may serve as a myeloid downregulatory signal.

A DNA sequence encoding the extracellular domain (Met 1-Pro 238) of mouse CD200R1 (NP_067300.1) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse CD200 at 1 μg/ml (100 μl/well) can bind mouse CD200R1/Fc with a linear range of 1.5-200 ng/ml.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The recombinant mouse CD200R1/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 462 amino acids and has a predicted molecular mass of 51.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmCD200R1/Fc monomer is approximately 90-100 kDa due to glycosylation.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.