TNFR-2 / CD120b Protein, Mouse, Recombinant (hFc Tag)

Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD120b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD120b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD120b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

CD120b Protein, Mouse; p75 Protein, Mouse; TNF-alphaR2 Protein, Mouse; TNF-R-II Protein, Mouse; TNF-R2 Protein, Mouse; TNF-R75 Protein, Mouse; TNFalpha-R2 Protein, Mouse; TNFBR Protein, Mouse; Tnfr-1 Protein, Mouse; Tnfr2 Protein, Mouse; TNFR80 Protein, Mouse; TNFRII Protein, Mouse

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The EDfor this effect is typically 20-80 ng/mL in the presence of 0.5 ng/mL of Recombinant Mouse TNFα.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The recombinant mouse TNFRSF1B/Fc is a disulfide-linked homodimer. The reduced monomer consists of 477 amino acids and has a predicted molecular mass of 52.3 kDa. In SDS-PAGE under reducing conditions, rm TNFRSF1B/Fc monomer migrates as an approximately 65 kDa band due to glycosylation.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

A DNA sequence encoding the extracellular domain of mouse TNFRSF1B (NP_035740.2) (Met 1-Gly 258) was fused with the Fc region of human IgG1 at the C-terminus.