EPOR Protein, Mouse, Recombinant (hFc Tag)

Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR/EPO Receptor) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR protein is a type a… single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR signaling prevents neuronal death and ischemic injury. Recent studies have shown that EPO and EPOR protein may be involved in carcinogenesis, angiogenesis, and invasion.

1. Measured by its ability to inhibit EPO-dependent proliferation of TF-1 human erythroleukemic cells. The EDfor this effect is typically 0.05-0.2 μg/mL in the presence of 16 ng/mL Recombinant mouse EPO. 2. Measured by its binding ability in a functional ELISA. 3. Immobilized mouse EPO-His (Cat:PM53458M2) at 10μg/mL (100 μL/well) can bind mouse EPOR-Fc. The ECof mouse EPOR-Fc is 0.06-0.13μg/mL.

Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, pH 7.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The recombinant mouse EPOR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 463 amino acids and has a predicted molecular mass of 51.4 kDa. The apparent molecular mass of the protein is approximately 58.6 kDa in SDS-PAGE under reducing conditions due to glycosylation.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.