CD79 Protein, Mouse, Recombinant (His Tag)
Product Specifications
Synonyms
Ig-alpha Protein, Mouse; Iga Protein, Mouse; Igalpha Protein, Mouse; Ly-54 Protein, Mouse; Ly54 Protein, Mouse; mb-1 Protein, Mouse
Gene Name
CD79a molecule, immunoglobulin-associated alpha
Expression System
HEK293 Cells
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Molecular Weight
The recombinant mouse CD79A & mouse CD79B heterodimer comprises 264 (120+144) amino acids and has a calculated molecular mass of 30.3 (13.8+ 16.5) kDa. The apparent molecular mass of the recombinant protein is approximately 34.6, 28.3 and 24.1 kDa respectively in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PM53144M2.pdf
Symbol
CD79A & CD79B
Species
Mouse
Protein ID
NP_031681.2&NP_031681.2
Overview
A DNA sequence encoding the mouse CD79A (NP_031681.2) (Met1-Arg137) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding mouse CD79B (NP_031681.2) (Met1-Asp158) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD79A & CD79B heterodimer was purified.
AA Sequence
Met1-Arg137 & Met1-Asp158
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/NP_031681.2&NP_031681.2
Available Sizes
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